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Image Search Results
Journal: Analytical and Bioanalytical Chemistry
Article Title: Spectrally separated dual-label upconversion luminescence lateral flow assay for cancer-specific STn-glycosylation in CA125 and CA15-3
doi: 10.1007/s00216-024-05275-z
Figure Lengend Snippet: Working principle of the spectrally separated dual-label LFA. Antibodies recognizing cancer-specific STn-glycosylation are immobilized on the test line (T). STn-glycovariants of cancer-associated biomarkers CA125 and CA15-3 are detected with erbium (Er 3+ ) and thulium (Tm 3+ ) doped UCNPs conjugated with antibodies against the protein epitopes. Illustration created with BioRender
Article Snippet:
Techniques: Glycoproteomics
Journal: Analytical and Bioanalytical Chemistry
Article Title: Spectrally separated dual-label upconversion luminescence lateral flow assay for cancer-specific STn-glycosylation in CA125 and CA15-3
doi: 10.1007/s00216-024-05275-z
Figure Lengend Snippet: Measurement of CA125 (erbium emission at 540 nm) with CA125-STn LFA (black), CA15-3-STn LFA (blue), and dual-label LFA (purple). Liver cirrhosis (LC) ascites fluid samples ( n = 3) were used as negative controls and they were assayed with ovarian cancer (OvCa)–positive ascites fluid samples ( n = 10)
Article Snippet:
Techniques:
Journal: Analytical and Bioanalytical Chemistry
Article Title: Spectrally separated dual-label upconversion luminescence lateral flow assay for cancer-specific STn-glycosylation in CA125 and CA15-3
doi: 10.1007/s00216-024-05275-z
Figure Lengend Snippet: Measurement of CA15-3 (thulium emission at 450 nm) with CA125-STn LFA (black), CA15-3-STn LFA (blue), and dual-label LFA (purple). Liver cirrhosis (LC) ascites fluid samples ( n = 3) were used as negative controls and they were assayed with ovarian cancer (OvCa)–positive ascites fluid samples ( n = 10)
Article Snippet:
Techniques:
Journal: Analytical and Bioanalytical Chemistry
Article Title: Spectrally separated dual-label upconversion luminescence lateral flow assay for cancer-specific STn-glycosylation in CA125 and CA15-3
doi: 10.1007/s00216-024-05275-z
Figure Lengend Snippet: Method comparison between the dual-label LFA and in-house well-based assay for the detection of CA125-STn ( A ) ( R 2 = 0.77) and CA15-3-STn ( B ) ( R 2 = 0.85). The method comparison was done with the ovarian cancer ascetic fluid samples ( n = 10)
Article Snippet:
Techniques: Comparison
Journal: Analytical and Bioanalytical Chemistry
Article Title: Spectrally separated dual-label upconversion luminescence lateral flow assay for cancer-specific STn-glycosylation in CA125 and CA15-3
doi: 10.1007/s00216-024-05275-z
Figure Lengend Snippet: Calibration curve for the dual-label LFA with CA125-STn calibrator. The calibration curves were assayed with CA125-STn calibrator diluted either in buffer (black) or in liver cirrhosis (LC) ascites fluid pool (purple). The analytical sensitivities were 1.8 U/ml and 3.6 U/ml, respectively (dashed lines)
Article Snippet:
Techniques:
Journal: Analytical and Bioanalytical Chemistry
Article Title: Spectrally separated dual-label upconversion luminescence lateral flow assay for cancer-specific STn-glycosylation in CA125 and CA15-3
doi: 10.1007/s00216-024-05275-z
Figure Lengend Snippet: Comparison of untreated samples and samples spiked with 1000 U/ml CA125-STn antigen to study anti-STn test line saturation with CA125 when CA15-3 is measured. A Effect of spiked CA125-STn in CA15-3 measurement. B Effect of spiked CA125-STn in CA125 measurement
Article Snippet:
Techniques: Comparison
Journal: International journal of cancer
Article Title: Ovarian Cancer Early Detection by Circulating CA125 in the context of Anti-CA125 Autoantibody Levels: Results from the EPIC cohort
doi: 10.1002/ijc.31164
Figure Lengend Snippet: (a) LOESS plot showing distribution of log-transformed CA125 among controls [blue] and, among cases, with cases classified in tertiles of anti-CA125 antibody levels. Tertiles of antibody level are represented by green (tertile 1; lowest), pink (tertile 2), and red (tertile 3; highest); (b) aAUC for CA125 by antibody tertiles for cases diagnosed within 2 years of blood collection (c) and between 2 and 4 years of blood collection. Phet represents the heterogeneity between aAUCs in the first and third tertiles.
Article Snippet: CA125 antigen and
Techniques: Transformation Assay